{"id":605,"date":"2021-10-01T16:08:34","date_gmt":"2021-10-01T23:08:34","guid":{"rendered":"https:\/\/luceome.com\/?page_id=605"},"modified":"2021-10-19T11:04:30","modified_gmt":"2021-10-19T18:04:30","slug":"cell-free-kinaseseeker-assays","status":"publish","type":"page","link":"https:\/\/luceome.com\/kinase-profiling-services\/cell-free-kinaseseeker-assays\/","title":{"rendered":"Cell-free KinaseSeeker&#x2122; Assays"},"content":{"rendered":"<p><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_5 1_5 fusion-flex-column fusion-no-small-visibility fusion-no-medium-visibility\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#efeeea;padding: 0px 0px 0px 0px;\"><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-0{width:20% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-0 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-0{width:100% !important;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-0{width:100% !important;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_5 1_5 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#efeeea;padding: 90px 30px 0px 15px;\"><div class=\"fusion-text fusion-text-1\"><h3 data-fontsize=\"32\" data-lineheight=\"32px\" class=\"fusion-responsive-typography-calculated\" style=\"--fontSize:32; line-height: 1;\"><span style=\"color: #3d4a6f;\">Kinase Profiling Panels<\/span><\/h3>\n<\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-0 fusion_builder_column_inner_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-1{border-radius:2px 2px 2px 2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-1 fusion-button-span-yes fusion-button-default-type\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/luceome.com\/wp-content\/uploads\/2018\/01\/Full-Length-Kinase-Panel.pdf\"><span class=\"fusion-button-text\">Full-Length Kinase Panel<\/span><\/a><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-0{width:50% !important;margin-top : 0px;margin-bottom : 10px;}.fusion-builder-nested-column-0 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 10px;padding-bottom : 0 !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-0{width:100% !important;order : 0;}.fusion-builder-nested-column-0 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-0{width:100% !important;order : 0;}.fusion-builder-nested-column-0 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-1 fusion_builder_column_inner_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-2{border-radius:2px 2px 2px 2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-2 fusion-button-span-yes fusion-button-default-type\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/luceome.com\/wp-content\/uploads\/2018\/01\/Tyrosine-Kinase-Panel.pdf\"><span class=\"fusion-button-text\">Tyrosine Kinase Panel<\/span><\/a><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-1{width:50% !important;margin-top : 0px;margin-bottom : 10px;}.fusion-builder-nested-column-1 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0 !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-1{width:100% !important;order : 0;}.fusion-builder-nested-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-1{width:100% !important;order : 0;}.fusion-builder-nested-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-2 fusion_builder_column_inner_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 10px 0px;\"><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-3{border-radius:2px 2px 2px 2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-3 fusion-button-span-yes fusion-button-default-type\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/luceome.com\/wp-content\/uploads\/2016\/09\/KinaseSeeker_MixedPanel.pdf\"><span class=\"fusion-button-text\">Mixed Kinase Panel<\/span><\/a><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-2{width:50% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-nested-column-2 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 10px;padding-bottom : 10px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-2{width:100% !important;order : 0;}.fusion-builder-nested-column-2 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-2{width:100% !important;order : 0;}.fusion-builder-nested-column-2 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-3 fusion_builder_column_inner_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 10px 0px;\"><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-4{border-radius:2px 2px 2px 2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-4 fusion-button-span-yes fusion-button-default-type\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker_Plasmodium-falciparum-Panel_11.pdf\"><span class=\"fusion-button-text\">Malarial Kinase Panel<\/span><\/a><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-3{width:50% !important;margin-top : 0px;margin-bottom : 15px;}.fusion-builder-nested-column-3 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 10px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-3{width:100% !important;order : 0;}.fusion-builder-nested-column-3 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-3{width:100% !important;order : 0;}.fusion-builder-nested-column-3 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-4 fusion_builder_column_inner_1_1 1_1 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-5{border-radius:2px 2px 2px 2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-5 fusion-button-span-yes fusion-button-default-type\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker_CompletePanel415.pdf\"><span class=\"fusion-button-text\">Complete Panel of 415 Kinases<\/span><\/a><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-4{width:100% !important;margin-top : 0px;margin-bottom : 10px;}.fusion-builder-nested-column-4 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-4{width:100% !important;order : 0;}.fusion-builder-nested-column-4 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-4{width:100% !important;order : 0;}.fusion-builder-nested-column-4 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><\/div><div class=\"fusion-text fusion-text-2\"><h4 class=\"fusion-responsive-typography-calculated\" style=\"--fontsize: 16; line-height: 1.5; --minfontsize: 16;\" data-fontsize=\"16\" data-lineheight=\"24px\"><span style=\"color: #3d4a6f;\"><b>Applications:<\/b><\/span><\/h4>\n<ul>\n<li>Rapidly screen test compounds or compound libraries at single concentration<\/li>\n<li>Quantitate inhibition in dose response titrations, measure IC50s<\/li>\n<li>Measure selectivity of an inhibitor against a kinase profiling panel<\/li>\n<li>Measure the impact of full-length vs kinase domain constructs on test compounds<\/li>\n<\/ul>\n<\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><\/div><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-6{border-radius:2px 2px 2px 2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-6 fusion-button-default-span fusion-button-default-type\" target=\"_self\" href=\"https:\/\/luceome.com\/contact-us\/\"><span class=\"fusion-button-text\">Request a Quote<\/span><\/a><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-1{width:20% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-1 > .fusion-column-wrapper {padding-top : 90px !important;padding-right : 30px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 15px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-1{width:100% !important;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-1{width:100% !important;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-2 fusion_builder_column_2_5 2_5 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#cccccc;padding: 90px 0px 0px 30px;\"><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-5 fusion_builder_column_inner_1_1 1_1 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 30px 0px 30px;\"><div class=\"fusion-text fusion-text-3\"><h3><span style=\"color: #3d4a6f;\">Cell-free KinaseSeeker&#x2122; Assays<\/span><\/h3>\n<p>Luceome offers two assay formats for evaluating kinase inhibitors. The Cell-free KinaseSeeker assay uses the proprietary <a href=\"https:\/\/luceome.com\/kinaseseeker-technology\/\">three-hybrid split-luciferase technology<\/a>, where the two luciferase fragment-fusion proteins are expressed in rabbit reticulocyte lysate and the reassembly of split-luciferase, mediated by a CID-probe, takes place in this cell-free system. Competitive displacement of the CID-probe by a kinase inhibitor is measured by a change in luminescence signal. The assay is a homogeneous binding assay, which enables rapid kinase profiling in a high-throughput format.<\/p>\n<\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;width:100%;\"><\/div><div ><span class=\" fusion-imageframe imageframe-none imageframe-1 hover-type-none\"><a href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Cell-free-KinaseSeeker-assay.png\" class=\"fusion-lightbox\" data-rel=\"iLightbox[89092eda7f531723c5e]\" data-title=\"KinaseSeeker Assay Principle\" title=\"Cell-free-KinaseSeeker-assay-2\"><img decoding=\"async\" width=\"1024\" height=\"233\" alt=\"Cell-free-KinaseSeeker-assay-2\" src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Cell-free-KinaseSeeker-assay-2.png\" data-orig-src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Cell-free-KinaseSeeker-assay-2-1024x233.png\" class=\"lazyload img-responsive wp-image-706\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271200%27%20height%3D%27273%27%20viewBox%3D%270%200%201200%20273%27%3E%3Crect%20width%3D%271200%27%20height%3D%27273%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-srcset=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Cell-free-KinaseSeeker-assay-2-200x46.png 200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Cell-free-KinaseSeeker-assay-2-400x91.png 400w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Cell-free-KinaseSeeker-assay-2-600x137.png 600w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Cell-free-KinaseSeeker-assay-2-800x182.png 800w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Cell-free-KinaseSeeker-assay-2.png 1200w\" data-sizes=\"auto\" data-orig-sizes=\"(max-width: 1024px) 100vw, (max-width: 640px) 100vw, 1024px\" \/><\/a><\/span><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;width:100%;\"><\/div><div class=\"fusion-text fusion-text-4\"><p>Increasing examples have shown that regulatory domains outside of the kinase domain can greatly influence the activation state of the kinase and consequently impact inhibitor binding. The KinaseSeeker<sup>&#x2122;<\/sup>\u00a0assay platform is highly amenable to full-length\/intracellular domain testing. These full-length and intracellular-domain containing kinases provide for numerous opportunities to investigate autoinhibited states, conformation-selective and allosteric inhibitors that otherwise would not be available in a standard kinase panel. Luceome has over 90 such constructs (across multiple kinase families) available for profiling in the cell-free format.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-5{width:100% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-nested-column-5 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 30px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 30px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-5{width:100% !important;order : 0;}.fusion-builder-nested-column-5 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-5{width:100% !important;order : 0;}.fusion-builder-nested-column-5 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-6 fusion_builder_column_inner_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-5\"><p style=\"text-align: center;\"><b>A<\/b> Type II inhibitor PDGFRa<\/p>\n<\/div><div style=\"text-align:center;\"><span class=\" fusion-imageframe imageframe-none imageframe-2 hover-type-none\"><a href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2.png\" class=\"fusion-lightbox\" data-rel=\"iLightbox[ad19712c3abdc15227f]\" data-title=\"Type II inhibitor PDGFRA\" title=\"Type II inhibitor PDGFRA\"><img decoding=\"async\" width=\"300\" height=\"267\" alt=\"Type II inhibitor PDGFRA\" src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2.png\" data-orig-src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2-300x267.png\" class=\"lazyload img-responsive wp-image-635\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271584%27%20height%3D%271408%27%20viewBox%3D%270%200%201584%201408%27%3E%3Crect%20width%3D%271584%27%20height%3D%271408%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-srcset=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2-200x178.png 200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2-400x356.png 400w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2-600x533.png 600w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2-800x711.png 800w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2-1200x1067.png 1200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeII-inhibitor_PDGFRA_2.png 1584w\" data-sizes=\"auto\" data-orig-sizes=\"(max-width: 1024px) 100vw, (max-width: 640px) 100vw, 800px\" \/><\/a><\/span><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-6{width:50% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-nested-column-6 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-6{width:100% !important;order : 0;}.fusion-builder-nested-column-6 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-6{width:100% !important;order : 0;}.fusion-builder-nested-column-6 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-7 fusion_builder_column_inner_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-6\"><p style=\"text-align: center;\"><b>B<\/b> Type I inhibitor LCK<\/p>\n<\/div><div style=\"text-align:center;\"><span class=\" fusion-imageframe imageframe-none imageframe-3 hover-type-none\"><a href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK-1024x910.png\" class=\"fusion-lightbox\" data-rel=\"iLightbox[f90d686749a3e2ff97b]\" data-title=\"Type I inhibitor LCK\" title=\"Type I inhibitor LCK\"><img decoding=\"async\" width=\"300\" height=\"267\" alt=\"Type I inhibitor LCK\" src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK.png\" data-orig-src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK-300x267.png\" class=\"lazyload img-responsive wp-image-636\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271584%27%20height%3D%271408%27%20viewBox%3D%270%200%201584%201408%27%3E%3Crect%20width%3D%271584%27%20height%3D%271408%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-srcset=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK-200x178.png 200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK-400x356.png 400w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK-600x533.png 600w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK-800x711.png 800w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK-1200x1067.png 1200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/TypeI-inhibitor_LCK.png 1584w\" data-sizes=\"auto\" data-orig-sizes=\"(max-width: 1024px) 100vw, (max-width: 640px) 100vw, 800px\" \/><\/a><\/span><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-7{width:50% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-nested-column-7 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-7{width:100% !important;order : 0;}.fusion-builder-nested-column-7 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-7{width:100% !important;order : 0;}.fusion-builder-nested-column-7 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;width:100%;\"><\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-8 fusion_builder_column_inner_1_1 1_1 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 30px 0px 30px;\"><div class=\"fusion-text fusion-text-7\"><p>(<b>A<\/b>) PDGFRa binding to a Type II inhibitor. Type II inhibitor binds the autoinhibited, juxtamembrane containing PDGFRa-intracellular module with reduced affinity (IC<sub>50<\/sub>\u00a0&gt; 10 \u00b5M), compared to the PDGFRa-kinase domain (IC<sub>50<\/sub>\u00a0= 44 nM). (<b>B<\/b>) LCK binding to a Type I inhibitor. A dramatic increase in binding potency is observed with the LCK-kinase domain (IC<sub>50<\/sub>\u00a0= 0.14 nM) over the SH2-SH3 domain containing LCK-full length construct (IC<sub>50<\/sub>\u00a0= 32 nM).<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-8{width:100% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-nested-column-8 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 30px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 30px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-8{width:100% !important;order : 0;}.fusion-builder-nested-column-8 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-8{width:100% !important;order : 0;}.fusion-builder-nested-column-8 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-2{width:40% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-2 > .fusion-column-wrapper {padding-top : 90px !important;padding-right : 0 !important;margin-right : 0px;padding-bottom : 0 !important;padding-left : 30px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-2{width:100% !important;}.fusion-builder-column-2 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-2{width:100% !important;}.fusion-builder-column-2 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-3 fusion_builder_column_1_5 1_5 fusion-flex-column fusion-no-small-visibility fusion-no-medium-visibility\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#cccccc;padding: 0px 0px 0px 0px;\"><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-3{width:20% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-3 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0 !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-3{width:100% !important;}.fusion-builder-column-3 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-3{width:100% !important;}.fusion-builder-column-3 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-1{ padding-top : 0px;margin-top : 0px;padding-right : 0px;padding-bottom : 0px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-2 fusion-parallax-none nonhundred-percent-fullwidth non-hundred-percent-height-scrolling fusion-equal-height-columns lazyload\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: repeat;padding-top:0px;padding-right:0px;padding-bottom:60px;padding-left:0px;margin-bottom: 0px;margin-top: 0px;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" data-bg=\"https:\/\/luceome.com\/wp-content\/uploads\/2018\/07\/linnen-bg.jpeg\" ><div class=\"fusion-builder-row fusion-row\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-4 fusion_builder_column_1_6 1_6 fusion-one-sixth fusion-column-first fusion-no-small-visibility\" style=\"width:16.666666666667%;width:calc(16.666666666667% - 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