{"id":607,"date":"2021-10-01T16:08:56","date_gmt":"2021-10-01T23:08:56","guid":{"rendered":"https:\/\/luceome.com\/?page_id=607"},"modified":"2022-02-28T11:57:46","modified_gmt":"2022-02-28T18:57:46","slug":"cell-based-kinaseseeker-te-assays","status":"publish","type":"page","link":"https:\/\/luceome.com\/kinase-profiling-services\/cell-based-kinaseseeker-te-assays\/","title":{"rendered":"Cell-based KinaseSeeker-TE&#x2122; Assays"},"content":{"rendered":"<p><div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container hundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:#eae9e9;border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_5 1_5 fusion-flex-column fusion-no-small-visibility fusion-no-medium-visibility\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#efeeea;padding: 0px 0px 0px 0px;\"><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-0{width:20% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-0 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-0{width:100% !important;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-0{width:100% !important;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_1_5 1_5 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#efeeea;padding: 90px 30px 0px 15px;\"><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:30px;width:100%;\"><\/div><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-1{border-radius:2px 2px 2px 2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-1 fusion-button-default-span fusion-button-default-type\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE_37-kinases.pdf\"><span class=\"fusion-button-text\">Cell-based KinaseSeeker-TE Panel<\/span><\/a><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;width:100%;\"><\/div><div class=\"fusion-text fusion-text-1\"><h4 class=\"fusion-responsive-typography-calculated\" style=\"--fontsize: 16; line-height: 1.5; --minfontsize: 16;\" data-fontsize=\"16\" data-lineheight=\"24px\"><span style=\"color: #3d4a6f;\"><b>Applications:<\/b><\/span><\/h4>\n<ul>\n<li>Measure cellular permeability and target engagement for a test compound<\/li>\n<li>Measure cellular potency and compare with in vitro biochemical potency<\/li>\n<li>Measure irreversible mode of inhibition for a test compound<\/li>\n<li>Measure potency for a prodrug inside cells<\/li>\n<li>Screen compound sets against a specific target kinase inside cells<\/li>\n<li>Measure selectivity inside cell for a test compound by using a kinase profiling panel<\/li>\n<\/ul>\n<\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><\/div><div style=\"text-align:center;\"><style>.fusion-body .fusion-button.button-2{border-radius:2px 2px 2px 2px;}<\/style><a class=\"fusion-button button-flat fusion-button-default-size button-default button-2 fusion-button-default-span fusion-button-default-type\" target=\"_self\" href=\"https:\/\/luceome.com\/contact-us\/\"><span class=\"fusion-button-text\">Request a Quote<\/span><\/a><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;margin-bottom:15px;width:100%;\"><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-1{width:20% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-1 > .fusion-column-wrapper {padding-top : 90px !important;padding-right : 30px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 15px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-1{width:100% !important;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-1{width:100% !important;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-2 fusion_builder_column_2_5 2_5 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#cccccc;padding: 90px 0px 0px 30px;\"><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-0 fusion_builder_column_inner_1_1 1_1 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 30px 0px 30px;\"><div class=\"fusion-text fusion-text-2\"><h3 class=\"fusion-responsive-typography-calculated\" style=\"--fontsize: 32; line-height: 1;\" data-fontsize=\"32\" data-lineheight=\"32px\"><span style=\"color: #3d4a6f;\">Cell-based KinaseSeeker-TE&#x2122; Assays<\/span><\/h3>\n<p>Target engagement (TE) assays are desirable for the generation of lead candidates and chemical probes. TE assays report on the direct binding of a drug to the target protein in a complex cellular environment. Luceome offers a wide range of cell-based TE assays for kinases using our popular <a href=\"https:\/\/luceome.com\/kinaseseeker-technology\/\">KinaseSeeker platform technology<\/a>. These TE assays allow measurement of compound binding to their specific target kinase inside a cell. Compounds can be screened and profiled for selectivity and their potencies and compared to in vitro assays.<\/p>\n<\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;width:100%;\"><\/div><div ><span class=\" fusion-imageframe imageframe-none imageframe-1 hover-type-none\"><a href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE-assay2-1024x334.png\" class=\"fusion-lightbox\" data-rel=\"iLightbox[773cddbd350d9cc5dc4]\" data-title=\"KinaseSeeker-TE Assay Principle\" title=\"KinaseSeeker-TE Assay Principle\"><img decoding=\"async\" width=\"1024\" height=\"334\" alt=\"cell based kinase assays\" src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE-assay2.png\" data-orig-src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE-assay2-1024x334.png\" class=\"lazyload img-responsive wp-image-621\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%272935%27%20height%3D%27957%27%20viewBox%3D%270%200%202935%20957%27%3E%3Crect%20width%3D%272935%27%20height%3D%27957%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-srcset=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE-assay2-200x65.png 200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE-assay2-400x130.png 400w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE-assay2-600x196.png 600w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE-assay2-800x261.png 800w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/KinaseSeeker-TE-assay2-1200x391.png 1200w\" data-sizes=\"auto\" data-orig-sizes=\"(max-width: 1024px) 100vw, (max-width: 640px) 100vw, 1024px\" \/><\/a><\/span><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;width:100%;\"><\/div><div class=\"fusion-text fusion-text-3\"><p>In the Cell-based KinaseSeeker-TE&#x2122; assay, the target kinase is expressed as a fusion protein to a luciferase fragment. Binding of a cell-permeable CID-probe to the target kinase brings about reassembly of the two complementary luciferase fragments thereby restoring luciferase activity, resulting in luminescence. Competitive displacement of the CID-probe by a test compound can be quantified in a dose dependent fashion by measuring loss of luminescence signal.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-0{width:100% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-nested-column-0 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 30px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 30px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-0{width:100% !important;order : 0;}.fusion-builder-nested-column-0 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-0{width:100% !important;order : 0;}.fusion-builder-nested-column-0 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-1 fusion_builder_column_inner_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-4\"><p style=\"text-align: center;\"><b>A<\/b> Reversible Inhibition of BTK with Das<\/p>\n<\/div><div style=\"text-align:center;\"><span class=\" fusion-imageframe imageframe-none imageframe-2 hover-type-none\"><a href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Reversible-.png\" class=\"fusion-lightbox\" data-rel=\"iLightbox[3784c327b1cce09c282]\" data-title=\"A Reversible Inhibition of BTK with Das\" title=\"A Reversible Inhibition of BTK with Das\"><img decoding=\"async\" width=\"300\" height=\"203\" alt=\"A Reversible Inhibition of BTK with Das\" src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Reversible-.png\" data-orig-src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Reversible--300x203.png\" class=\"lazyload img-responsive wp-image-626\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27704%27%20height%3D%27476%27%20viewBox%3D%270%200%20704%20476%27%3E%3Crect%20width%3D%27704%27%20height%3D%27476%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-srcset=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Reversible--200x135.png 200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Reversible--400x270.png 400w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Reversible--600x406.png 600w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Reversible-.png 704w\" data-sizes=\"auto\" data-orig-sizes=\"(max-width: 1024px) 100vw, (max-width: 640px) 100vw, 300px\" \/><\/a><\/span><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-1{width:50% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-nested-column-1 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-1{width:100% !important;order : 0;}.fusion-builder-nested-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-1{width:100% !important;order : 0;}.fusion-builder-nested-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-2 fusion_builder_column_inner_1_2 1_2 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-5\"><p style=\"text-align: center;\"><b>B<\/b> Irreversible Inhibition of BTK with Ibru<\/p>\n<\/div><div style=\"text-align:center;\"><span class=\" fusion-imageframe imageframe-none imageframe-3 hover-type-none\"><a href=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Irreversible-.png\" class=\"fusion-lightbox\" data-rel=\"iLightbox[5197e9682ebd4d47688]\" data-title=\"B Irreversible Inhibition of BTK with Ibru\" title=\"B Irreversible Inhibition of BTK with Ibru\"><img decoding=\"async\" width=\"300\" height=\"200\" alt=\"B Irreversible Inhibition of BTK with Ibru\" src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Irreversible-.png\" data-orig-src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Irreversible--300x200.png\" class=\"lazyload img-responsive wp-image-625\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27729%27%20height%3D%27486%27%20viewBox%3D%270%200%20729%20486%27%3E%3Crect%20width%3D%27729%27%20height%3D%27486%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-srcset=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Irreversible--200x133.png 200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Irreversible--400x267.png 400w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Irreversible--600x400.png 600w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/Irreversible-.png 729w\" data-sizes=\"auto\" data-orig-sizes=\"(max-width: 1024px) 100vw, (max-width: 640px) 100vw, 300px\" \/><\/a><\/span><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-2{width:50% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-nested-column-2 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-2{width:100% !important;order : 0;}.fusion-builder-nested-column-2 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-2{width:100% !important;order : 0;}.fusion-builder-nested-column-2 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:15px;width:100%;\"><\/div><div class=\"fusion-builder-row fusion-builder-row-inner fusion-row fusion-flex-align-items-stretch\" style=\"width:calc( 100% + 0px ) !important;max-width:calc( 100% + 0px ) !important;margin-left: calc(-0px \/ 2 );margin-right: calc(-0px \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column_inner fusion-builder-nested-column-3 fusion_builder_column_inner_1_1 1_1 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 30px 0px 30px;\"><div ><span class=\" fusion-imageframe imageframe-none imageframe-4 hover-type-none\"><img decoding=\"async\" width=\"628\" height=\"102\" title=\"cell-based-info-2\" src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/cell-based-info-2.png\" data-orig-src=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/cell-based-info-2.png\" alt class=\"lazyload img-responsive wp-image-858\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27628%27%20height%3D%27102%27%20viewBox%3D%270%200%20628%20102%27%3E%3Crect%20width%3D%27628%27%20height%3D%27102%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-srcset=\"https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/cell-based-info-2-200x32.png 200w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/cell-based-info-2-400x65.png 400w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/cell-based-info-2-600x97.png 600w, https:\/\/luceome.com\/wp-content\/uploads\/2021\/10\/cell-based-info-2.png 628w\" data-sizes=\"auto\" data-orig-sizes=\"(max-width: 1024px) 100vw, (max-width: 640px) 100vw, 628px\" \/><\/span><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-nested-column-3{width:100% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-nested-column-3 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 30px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 30px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-nested-column-3{width:100% !important;order : 0;}.fusion-builder-nested-column-3 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-nested-column-3{width:100% !important;order : 0;}.fusion-builder-nested-column-3 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-2{width:40% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-2 > .fusion-column-wrapper {padding-top : 90px !important;padding-right : 0 !important;margin-right : 0px;padding-bottom : 0 !important;padding-left : 30px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-2{width:100% !important;}.fusion-builder-column-2 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-2{width:100% !important;}.fusion-builder-column-2 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-3 fusion_builder_column_1_5 1_5 fusion-flex-column fusion-no-small-visibility fusion-no-medium-visibility\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;background-color:#cccccc;padding: 0px 0px 0px 0px;\"><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-3{width:20% !important;margin-top : 0px;margin-bottom : 0px;}.fusion-builder-column-3 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0 !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-3{width:100% !important;}.fusion-builder-column-3 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-3{width:100% !important;}.fusion-builder-column-3 > .fusion-column-wrapper {margin-right : 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